Heparinases I Applications

Some known applications of Heparinase I enzyme:

  • Production of low- and ultra-low molecular weight heparins (LMWH and ULMWH)
  • Characterization of heparin, heparan sulfate (HS), LMWH, and synthetic heparin pentasaccharides and oligosaccharides
  • Structural analyses of heparin, HS and LMWH
  • Depolymerization of heparin, HS and chemically modified heparins, and molecular weight profiling of heparins and HS
  • Quality control testing of heparin and heparin-derived products,
  • Quantification of contaminants in heparin such as over-sulfated chondroitin sulfate and persulfonated heparin
  • Quantification of process-related impurities
  • Glycobiology research
  • Coagulation and anticoagulation efficacy studies
  • Thromboelastography
  • Determination of the spacing between S-domains in the HS using the enzymatically digested fragment analysis of HS
  • Identification of the biological properties of HS that depend on the integrity of the S-domains
  • In vitro host-pathogen interactions in viral infections, in vitro virus-adhesion inhibition studies, cell culture experiments
  • In vitro virus plaque inhibition assays
  • In vivo inhibition studies of neovascularization and proliferation of capillary endothelial cells
  • Mass spectral analysis of heparins and heparan sulfates
  • Immobilization of heparinase I on to chromatography resins to build columns for LMWH production and analytical testing,
  • Cancer biology research
  • Circumventing the inhibitory effects of heparin in PCR, RT-PCR, real-time RT-qPCR reaction and Western Blot
  • In vitro histochemistry, immunohistochemistry, immunocytochemistry and flow cytometry

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