Some known applications of Heparinase I enzyme:

• Production of low- and ultra-low molecular weight heparins (LMWH and ULMWH)
• Characterization of heparin, heparan sulfate (HS), LMWH, and synthetic heparin pentasaccharides and oligosaccharides
• Structural analyses of heparin, HS and LMWH
• Depolymerization of heparin, HS and chemically modified heparins, and molecular weight profiling of heparins and HS
• Quality control testing of heparin and heparin-derived products,
• Quantification of contaminants in heparin such as over-sulfated chondroitin sulfate and persulfonated heparin
• Quantification of process-related impurities
• Glycobiology research
• Coagulation and anticoagulation efficacy studies
• Thromboelastography
• Determination of the spacing between S-domains in the HS using the enzymatically digested fragment analysis of HS
• Identification of the biological properties of HS that depend on the integrity of the S-domains
• In vitro host-pathogen interactions in viral infections, in vitro virus-adhesion inhibition studies, cell culture experiments
• In vitro virus plaque inhibition assays
• In vivo inhibition studies of neovascularization and proliferation of capillary endothelial cells
• Mass spectral analysis of heparins and heparan sulfates
• Immobilization of heparinase I on to chromatography resins to build columns for LMWH production and analytical testing,
• Cancer biology research
• Circumventing the inhibitory effects of heparin in PCR, RT-PCR, real-time RT-qPCR reaction and Western Blot
• In vitro histochemistry, immunohistochemistry, immunocytochemistry and flow cytometry