Flavobacterium Heparinase III

Heparinase III, also known as Heparin Lyase III or heparan sulfate lyase/eliminase or Heparitinase I is an endolytic enzyme. It cleaves selectively and exclusively the heparan sulfate via an elimination mechanism, undersulfated polysaccharide chains or acetylated or unsubstituted 1-4 linkages between hexosamines and glucuronic acid residues. The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. The enzyme is active only on heparan sulfate and does not cleave heparin.

Recombinant enzyme homologous to its native counterpart and expressed in the natural host, Pedobacter heparinus (formerly, Flavobacterium heparinum).

  • EC Number: 4.2.2.8
  • Purity: ≥ 95 % by reversed phase HPLC analysis
  • IBEX liquid heparinase III has optimal activity and stability for up to 30 months when stored frozen at -70°C
  • IBEX lyophilized heparinase I has optimal activity and stability for up to two years when stored at 2-8oC
  • Calcium dependent enzyme and has optimal activity in the presence of 1.5-5.0 mM CaCl2 . Highest activity observed when Ca+2 was at 10 mM

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Data Sheet - LIQUID
Safety Data Sheet - LIQUID

Data Sheet - LYOPHILIZED
Safety Data Sheet - Lyophilized