Heparinases in Research Applications
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Heparinases in Medical Testing Applications
In vitro neutralization of heparin in blood & plasma samples before analysis.
• Preparation of disaccharides of heparin & preparation of oligosaccharide libraries.
• Measurement of heparin in blood & plasma using the in vitro thromboelastography (TEG) tests.
• Coagulation & anticoagulation efficacy studies.
• Production of low- & ultra-low molecular weight heparins from unfractionated heparin & immobilization of heparinase I for such use.
• Structural analyses, mass spectral analysis & characterization of heparin, heparan sulfate (HS), low molecular weight heparins, & synthetic heparin pentasaccharides & oligosaccharides.
• Depolymerization of heparin, HS & chemically modified heparins, & molecular weight profiling of heparins.
• Quantification of contaminants in heparin such as oversulfated chondroitin sulfate & persulfonated heparin & quantification of process-related impurities.
• Glycobiology & cancer biology research.
• Identification of the biological properties of HS that depend on the integrity of the S-domains & determination of the spacing between S-domains.
• In vitro host-pathogen interactions in viral infections, virusadhesion inhibition studies, virus-plaque inhibition assays, cell culture experiments, etc.
• In vivo inhibition studies of neovascularization & proliferation of capillary endothelial cells.
• Circumventing the inhibitory effects of heparin in PCR, RTPCR, real-time RT-qPCR reaction & Western Blot.
• In vitro histochemistry, immunohistochemistry, immunocytochemistry & flow cytometry, etc.
Holding for future use:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140326/
https://www.sciencedirect.com/science/article/pii/S2214753516300055
https://pubmed.ncbi.nlm.nih.gov/8218862/
https://pubmed.ncbi.nlm.nih.gov/7943773/
https://pubmed.ncbi.nlm.nih.gov/9268178/
https://pubmed.ncbi.nlm.nih.gov/24461364/
PDFs from Paul
https://heparinase.com/wp-content/uploads/2022/09/anticoagulant_testing_maier_et_al_2021.pdf