Heparinases I Applications

Some known applications of Heparinase I enzyme:

• In vitro neutralization of heparin in blood & plasma samples before analysis.
• Preparation of disaccharides of heparin & the preparation of oligosaccharide libraries.
• Measurement of heparin in blood & plasma using the in vitro thromboelastography (TEG) tests. • Coagulation & anticoagulation efficacy studies.
• Production of low- & ultra-low molecular weight heparins (LMWH & ULMWH) from unfractionated heparin & immobilization of heparinase I for such use.
• In-process, quality control, & compendial testing of heparins, heparan sulfate (HS), heparin- & HS-derived products.
• Structural analysis, mass spectral analysis & characterization of heparin, heparan sulfate (HS), low molecular weight heparins, & synthetic heparin pentasaccharides & oligosaccharides.
• Depolymerization of heparin, HS & chemically modified heparins, & molecular weight profiling of heparins.
• Quantification of contaminants in heparin such as over-sulfated chondroitin sulfate & persulfonated heparin & quantification of process-related impurities. • Glycobiology & cancer biology research.
• Identification of the biological properties of HS that depend on the integrity of the S-domains & determination of the spacing between S-domains.
• In vitro host-pathogen interactions in viral infections, virusadhesion inhibition studies, virus-plaque inhibition assays, cell culture experiments, etc.
• In vivo inhibition studies of neovascularization & proliferation of capillary endothelial cells.
• Circumventing the inhibitory effects of heparin in PCR, RT-PCR, real-time RT-qPCR reaction& Western Blot.
• In vitro histochemistry, immunohistochemistry, immunocytochemistry & flow cytometry, etc

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