Heparinase III Applications
- Production of low- and ultra-low molecular weight heparins (LMWH and ULMWH)
- Characterization of heparin, heparan sulfate (HS), LMWH
- Compositional analysis of glycocalyx in tissues and on cells
- Depolymerization of heparin, HS and chemically modified heparins and molecular weight profiling of heparins and HS
- Production and isolation of oligosaccharides with novel sequences of GlcNH3+ residues
- Quality control testing of heparin and heparin-derived products,
- Quantification of contaminants in heparin such as over-sulfated chondroitin sulfate and persulfonated heparin
- Quantification of process-related impurities
- Glycobiology research,
- Determination of the spacing between S-domains in the HS using the enzymatically digested fragment analysis of HS
- Identification of the biological properties of HS that depend on the integrity of the S-domains
- In vitro host-pathogen interactions in viral infections, in vitro virus-adhesion inhibition studies, cell culture experiments
- In vitro virus plaque inhibition assays
- In vivo inhibition studies of neovascularization and proliferation of capillary endothelial cells
- Mass spectral analysis of heparins and heparan sulfates
- Cancer biology research
- In vitro histochemistry, immunohistochemistry, immunocytochemistry and flow cytometry
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Heparinase III has a variety of applications in the field of biochemistry and biotechnology. Some of the most common applications include:
- Glycosaminoglycan analysis: Heparinase III is often used to cleave heparan sulfate and heparin chains from proteoglycans, allowing for the analysis of the structure and composition of these molecules.
- Anticoagulant research: Heparinase III can be used to study the role of heparin and heparan sulfate in blood coagulation and thrombosis.
- Cell culture studies: Heparinase III is used to remove heparin or heparan sulfate from cell cultures, allowing researchers to study the effects of these molecules on cell behavior.
- Tissue engineering: Heparinase III is used to modify the surface of biomaterials to control cell adhesion and tissue formation.
- Drug development: Heparinase III can be used to modify the structure of heparin and heparan sulfate molecules for the development of new therapeutic agents.