Heparinase III Applications -

Heparinase III Applications

  • Production of low- and ultra-low molecular weight heparins (LMWH and ULMWH)
  • Characterization of heparin, heparan sulfate (HS), LMWH
  • Compositional analysis of glycocalyx in tissues and on cells
  • Depolymerization of heparin, HS and chemically modified heparins  and molecular weight profiling of heparins and HS
  • Production and isolation of oligosaccharides with novel sequences of GlcNH3+ residues
  • Quality control testing of heparin and heparin-derived products,
  • Quantification of contaminants in heparin such as over-sulfated chondroitin sulfate and persulfonated heparin
  • Quantification of process-related impurities
  • Glycobiology research,
  • Determination of the spacing between S-domains in the HS using the enzymatically digested fragment analysis of HS
  • Identification of the biological properties of HS that depend on the integrity of the S-domains
  • In vitro host-pathogen interactions in viral infections, in vitro virus-adhesion inhibition studies, cell culture experiments
  • In vitro virus plaque inhibition assays
  • In vivo inhibition studies of neovascularization and proliferation of capillary endothelial cells
  • Mass spectral analysis of heparins and heparan sulfates
  • Cancer biology research
  • In vitro histochemistry, immunohistochemistry, immunocytochemistry and flow cytometry

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Heparinase III has a variety of applications in the field of biochemistry and biotechnology. Some of the most common applications include:

  1. Glycosaminoglycan analysis: Heparinase III is often used to cleave heparan sulfate and heparin chains from proteoglycans, allowing for the analysis of the structure and composition of these molecules.
  2. Anticoagulant research: Heparinase III can be used to study the role of heparin and heparan sulfate in blood coagulation and thrombosis.
  3. Cell culture studies: Heparinase III is used to remove heparin or heparan sulfate from cell cultures, allowing researchers to study the effects of these molecules on cell behavior.
  4. Tissue engineering: Heparinase III is used to modify the surface of biomaterials to control cell adhesion and tissue formation.
  5. Drug development: Heparinase III can be used to modify the structure of heparin and heparan sulfate molecules for the development of new therapeutic agents.