Heparinase III Applications

• Production of low- and ultra-low molecular weight heparins (LMWH and ULMWH)
• Characterization of heparin, heparan sulfate (HS), LMWH
• Compositional analysis of glycocalyx in tissues and on cells
• Depolymerization of heparin, HS and chemically modified heparins  and molecular weight profiling of heparins and HS
• Production and isolation of oligosaccharides with novel sequences of GlcNH₃⁺ residues
• Quality control testing of heparin and heparin-derived products,
• Quantification of contaminants in heparin such as over-sulfated chondroitin sulfate and persulfonated heparin
• Quantification of process-related impurities
• Glycobiology research,
• Determination of the spacing between S-domains in the HS using the enzymatically digested fragment analysis of HS
• Identification of the biological properties of HS that depend on the integrity of the S-domains
• In vitro host-pathogen interactions in viral infections, in vitro virus-adhesion inhibition studies, cell culture experiments
• In vitro virus plaque inhibition assays
• In vivo inhibition studies of neovascularization and proliferation of capillary endothelial cells
• Mass spectral analysis of heparins and heparan sulfates
• Cancer biology research
• In vitro histochemistry, immunohistochemistry, immunocytochemistry and flow cytometry

Heparinase III has a variety of applications in the field of biochemistry and biotechnology. Some of the most common applications include:

1. Glycosaminoglycan analysis: Heparinase III is often used to cleave heparan sulfate and heparin chains from proteoglycans, allowing for the analysis of the structure and composition of these molecules.
2. Anticoagulant research: Heparinase III can be used to study the role of heparin and heparan sulfate in blood coagulation and thrombosis.
3. Cell culture studies: Heparinase III is used to remove heparin or heparan sulfate from cell cultures, allowing researchers to study the effects of these molecules on cell behavior.
4. Tissue engineering: Heparinase III is used to modify the surface of biomaterials to control cell adhesion and tissue formation.
5. Drug development: Heparinase III can be used to modify the structure of heparin and heparan sulfate molecules for the development of new therapeutic agents.