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In vitro neutralization of heparin in blood & plasma samples before analysis.
• Preparation of disaccharides of heparin & preparation of oligosaccharide libraries.
• Measurement of heparin in blood & plasma using the in vitro thromboelastography (TEG) tests.
• Coagulation & anticoagulation efficacy studies.
• Production of low- & ultra-low molecular weight heparins from unfractionated heparin & immobilization of heparinase I for such use.
• Structural analyses, mass spectral analysis & characterization of heparin, heparan sulfate (HS), low molecular weight heparins, & synthetic heparin pentasaccharides & oligosaccharides.
• Depolymerization of heparin, HS & chemically modified heparins, & molecular weight profiling of heparins.
• Quantification of contaminants in heparin such as oversulfated chondroitin sulfate & persulfonated heparin & quantification of process-related impurities.
• Glycobiology & cancer biology research.
• Identification of the biological properties of HS that depend on the integrity of the S-domains & determination of the spacing between S-domains.
• In vitro host-pathogen interactions in viral infections, virusadhesion inhibition studies, virus-plaque inhibition assays, cell culture experiments, etc.
• In vivo inhibition studies of neovascularization & proliferation of capillary endothelial cells.
• Circumventing the inhibitory effects of heparin in PCR, RTPCR, real-time RT-qPCR reaction & Western Blot.
• In vitro histochemistry, immunohistochemistry, immunocytochemistry & flow cytometry, etc.